Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
نویسندگان
چکیده
منابع مشابه
Detection of Rotavirus Using Padlock Probes and Rolling Circle Amplification
Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a no...
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Circularizing oligonucleotide probes (padlock probes) have the potential to detect sets of gene sequences with high specificity and excellent selectivity for sequence variants, but sensitivity of detection has been limiting. By using a rolling circle replication (RCR) mechanism, circularized but not unreacted probes can yield a powerful signal amplification. We demonstrate here that in order fo...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2015
ISSN: 1516-8913
DOI: 10.1590/s1516-8913201502762